05/18/05 10:56:40 PLONDRY Logs in [192.197.166.63]
05/18/05 10:56:51 PLONDRY Logs Out
05/18/05 17:08:22 jim Logs in [69.9.219.17]
05/18/05 17:08:26 jim Logs Out
05/18/05 19:08:27 jim Logs in [69.9.219.17]
05/18/05 19:08:30 jim Logs Out
05/18/05 19:24:21 heinrichthelma Logs in [68.226.107.114]
05/18/05 19:24:33 heinrichthelma Logs Out
05/18/05 19:30:46 Rachel_ Logs in [216.211.102.149]
05/18/05 19:32:16 Rachel_ Logs Out
05/18/05 19:32:40 Becca,IN Logs in [71.98.76.32]
05/18/05 19:32:44 Becca,IN Logs Out
05/18/05 19:34:53 jim Logs in [69.9.219.17]
05/18/05 19:34:56 jim Logs Out
05/18/05 19:40:31 Clare_KS Logs in [65.26.89.232]
05/18/05 19:40:40 Clare_KS Logs Out
05/18/05 19:47:00 Jana_in_Indiana Logs in [172.159.137.109]
05/18/05 19:49:52 Denise Logs in [204.50.10.10]
05/18/05 19:51:17 Denise Logs Out
05/18/05 19:54:40 adgjoan Logs in [12.203.138.143]
05/18/05 19:54:48 Jana_in_Indiana: HI Joan!
05/18/05 19:55:27 adgjoan: Hi, Jana are you waiting for everyone else to join the chat?
05/18/05 19:55:55 Jana_in_Indiana: yes, LOL, I see Denise was here & left,, I missed her, was copying a file
05/18/05 19:56:38 Vivian__tn Logs in [63.17.217.105]
05/18/05 19:56:44 Jana_in_Indiana: Hi vivian!
05/18/05 19:57:03 Jana_in_Indiana: Joan, what does the adg stand for?
05/18/05 19:57:12 adgjoan: I probably will not stay long either causr I can not stay up this late at night
05/18/05 19:57:25 Vivian__tn: hI jANA, GUESS i'M A LITTLE EARLY
05/18/05 19:57:30 Jana_in_Indiana: the chat log will be posted so you can read anything you miss
05/18/05 19:57:39 Jana_in_Indiana: only 4 minutes early!
05/18/05 19:57:50 Clare_KS Logs in [65.26.89.232]
05/18/05 19:57:56 Clare_KS: Hellody
05/18/05 19:57:56 Jana_in_Indiana: we do have many that arrive late, LOL
05/18/05 19:57:59 Jana_in_Indiana: Hi Clare
05/18/05 19:58:35 adgjoan: am very lazy so when I needed to come up with a name I just typed every other letter on the key board and put joan behind it.
05/18/05 19:58:38 Clare_KS: My hard drive crashed, I've been without my computer for 2 days!!!
05/18/05 19:58:57 Jana_in_Indiana: LOL, good idea Joan!  ;)
05/18/05 19:59:00 Denise Logs in [204.50.10.10]
05/18/05 19:59:00 Clare_KS: Now I have a new one, 4 times bigger :)
05/18/05 19:59:08 Jana_in_Indiana: welcome back Denise!
05/18/05 19:59:15 Jana_in_Indiana: sorry I missed you before
05/18/05 19:59:28 Denise: Hi Jana, everyone.
05/18/05 19:59:29 Jana_in_Indiana: that would have drove me crazy Clare
05/18/05 19:59:42 Clare_KS: It did me...the last time it was 6 WEEKS.
05/18/05 19:59:44 Vivian__tn: AM SO GLAD YOU ARE GOING TO POST THE CHAT, i AM NOT VERY GOOD STAYING UP LATE
05/18/05 19:59:56 Jana_in_Indiana: is this Denise from AVSB?
05/18/05 20:00:19 Denise Logs Out
05/18/05 20:00:23 Jana_in_Indiana: yes, everything is automatically logged to we can post it
05/18/05 20:00:37 Charlene_in_OK Logs in [68.12.244.136]
05/18/05 20:00:43 Jana_in_Indiana: Hi Charlene!
05/18/05 20:00:51 paula0103 Logs in [12.223.195.199]
05/18/05 20:00:57 Jana_in_Indiana: Hi Paula!
05/18/05 20:01:00 Charlene_in_OK: Hi Jana, hi Paula
05/18/05 20:01:12 paula0103: Hi Everyone!
05/18/05 20:01:15 Charlene_in_OK: I can't wait to hear all your information tonight.
05/18/05 20:01:25 Vivian__tn: jANA, i JUST WANTED TO TELL YOU HOW MUCH i AM ENJOYING THE CONVENTION PICTURES THEY ARE GREAT
05/18/05 20:01:37 Cheryl-TX Logs in [68.94.44.71]
05/18/05 20:01:39 Jana_in_Indiana: if anyone else has done TC, be sure to add your input!
05/18/05 20:01:44 Charlene_in_OK: Hi Cheryl
05/18/05 20:01:45 Jana_in_Indiana: Hi Cheryl!
05/18/05 20:01:59 Jana_in_Indiana: thanks Vivian!
05/18/05 20:02:06 violetangel Logs in [68.104.132.18]
05/18/05 20:02:20 Jana_in_Indiana: Hi Violetangel, name please?
05/18/05 20:02:32 Cheryl-TX: yall are gathering early
05/18/05 20:02:46 Clare_KS: Nope, it's past the hour :)
05/18/05 20:03:03 Charlene_in_OK: Hi Clare
05/18/05 20:03:11 Jana_in_Indiana: right Clare!  But, we seldome start on time, LOL
05/18/05 20:03:26 Clare_KS: I sit corrected.
05/18/05 20:03:31 Cheryl-TX: yes this is rare
05/18/05 20:03:45 Cheryl-TX: we dont really get going until the 1/2 hour
05/18/05 20:04:08 Clare_KS: My Gel2Root just came
05/18/05 20:04:14 sherry__(Mo) Logs in [172.136.149.62]
05/18/05 20:04:20 Jana_in_Indiana: Hi sherry!
05/18/05 20:04:32 Jana_in_Indiana: great Clare, let us know how it works for you
05/18/05 20:04:33 sherry__(Mo): Hi!!!
05/18/05 20:04:37 Charlene_in_OK: Where did you get it from Gel2Root?
05/18/05 20:05:00 Sarah Logs in [216.45.168.113]
05/18/05 20:05:08 Jana_in_Indiana: be sure to keep it in something with water in the bottom or it will dry out & get hard as a rock!
05/18/05 20:05:09 Charlene_in_OK: Hi Sherry and Sarah
05/18/05 20:05:12 Sarah: hey
05/18/05 20:05:14 Jana_in_Indiana: Hi Sarah!
05/18/05 20:05:17 Clare_KS: Charley's Greenhouse was the only place I could find.
05/18/05 20:05:37 Jana_in_Indiana: I have 1 other place, but they charge more
05/18/05 20:06:00 Jana_in_Indiana: I always get the 1 quart bottle, not the 6 packs -- they waste too much gel
05/18/05 20:06:08 Cheryl-TX: is gel2root like keki grow
05/18/05 20:06:24 Jana_in_Indiana: no, it's for rooting cuttings of almost anything, AV's too
05/18/05 20:06:34 Charlene_in_OK: Gotcha, I am going to try the water crystals, anyone know the url for Charleys
05/18/05 20:06:38 Jana_in_Indiana: go to www.gel2root.com for info
05/18/05 20:06:49 Cheryl-TX: i put some keki grow on my leaves last night
05/18/05 20:07:15 Jana_in_Indiana: http://www.charleysgreenhouse.com
05/18/05 20:07:15 violetangel: what does keki grow do.
05/18/05 20:07:28 Jana_in_Indiana: exactly how did you do it cheryl?
05/18/05 20:07:42 Jana_in_Indiana: stimulates suckers, used mostly on chimeras
05/18/05 20:08:06 Cheryl-TX: i am not really sure, tony sent me a little dab and i want to use  it and so i am putting it on every th\thing
05/18/05 20:08:09 Cheryl-TX: lol
05/18/05 20:08:15 adgjoan: is keki grow root hormone?
05/18/05 20:08:31 Cheryl-TX: well it is some kind of hormone
05/18/05 20:08:47 Jana_in_Indiana: it's a paste with hormones in it, to be applied to leaf bracts on bloom stems & necks to cause suckers
05/18/05 20:09:03 Charlene_in_OK: There was great information on it's use in RachelsReflections site
05/18/05 20:09:04 Cheryl-TX: i wanted to use it for bloom stem propagation on chimeras
05/18/05 20:09:40 Charlene_in_OK: All I read says that you use very very little
05/18/05 20:09:40 Jana_in_Indiana: http://www.orchidmall.com/hormones/index.htm
05/18/05 20:10:04 Carol_SK Logs in [142.165.188.200]
05/18/05 20:10:09 Rachel Logs in [216.211.102.149]
05/18/05 20:10:13 Charlene_in_OK: Hi Carol and Rachel
05/18/05 20:10:17 Carol_SK: Hi All
05/18/05 20:10:26 Rachel: Hello everyone
05/18/05 20:10:34 Jana_in_Indiana: Hi Carol & Rachel!
05/18/05 20:11:01 Jana_in_Indiana: right charlene, use only a tiny dab
05/18/05 20:11:15 Cheryl-TX: the room is getting full Jana maybe you can start telling us about your tissue culture
05/18/05 20:11:29 Jana_in_Indiana: it's made by a company in Canada, but I can't find my link
05/18/05 20:11:41 Jana_in_Indiana: ok, where do you want me to start?
05/18/05 20:11:51 Cheryl-TX: from the beginning
05/18/05 20:12:00 Carol_SK: lol
05/18/05 20:12:18 Jana_in_Indiana: I bought the basic kit from Kitchen Tissue Culture quite a while ago
05/18/05 20:12:27 jim Logs in [69.9.219.17]
05/18/05 20:12:41 Rachel: Hello Jim
05/18/05 20:12:45 Charlene_in_OK: Hi jim
05/18/05 20:12:47 jim: wow a few people here
05/18/05 20:12:51 jim: hi everyone
05/18/05 20:13:06 Jana_in_Indiana: http://www.kitchenculturekit.com/avleafdevelop.htm
05/18/05 20:13:12 Jana_in_Indiana: Hi Jim
05/18/05 20:13:13 Tony_IL Logs in [207.43.75.34]
05/18/05 20:13:17 Jana_in_Indiana: Hi Tony!
05/18/05 20:13:22 Rachel: Hello tony
05/18/05 20:13:31 Charlene_in_OK: Hi Tony
05/18/05 20:13:40 Tony_IL: hi everyone. Full house tonite
05/18/05 20:13:43 Jana_in_Indiana: then I got my daughter to agree to be the dirty person (the un-sterile one, LOL)
05/18/05 20:13:55 Jana_in_Indiana: we picked a date she be home & did it
05/18/05 20:14:18 Cheryl-TX: ok i need some more details
05/18/05 20:14:29 Carol_SK: KJana, how long of a 'proceedure' is it?
05/18/05 20:14:31 adgjoan: did what?
05/18/05 20:14:53 Jana_in_Indiana: I used a 10 gallon aquarium lying on its long side for a ‘clean box’ with a piece of plexi-glass over the upper 2/3 of the opening.
05/18/05 20:15:15 Jana_in_Indiana: .  It is VERY important to NOT have ANY air movement.  No fans, furnace, A/C, people moving, pets moving, doors opening or closing – nothing extra moving in your home when doing your culture.
05/18/05 20:15:31 Jana_in_Indiana: depends on how fast you work Carol!
05/18/05 20:15:49 Jana_in_Indiana: Air movement increases the likelihood of contamination.  I sterilized the culture media in a pressure cooker.  You can also use a microwave oven.
05/18/05 20:15:59 Carol_SK: laffin, couple of hours?  6 hours three days ??
05/18/05 20:16:22 Jana_in_Indiana: it was about 3 hours for me, I'm very slow!
05/18/05 20:16:26 Jana_in_Indiana: .  I grew all my cultures in baby food jars.  For ease of culturing, my daughter worked as the ‘dirty person’ (dirty means not sterile) and I was the ‘clean person’.
05/18/05 20:16:55 Jana_in_Indiana: Anngela did all the retrieving, soaking & labeling work.  I did the cutting, transfer, placement & sealing of the cultures.
05/18/05 20:17:04 Cheryl-TX: you sterilized  your self???????
05/18/05 20:17:31 jim: would this work with streps
05/18/05 20:17:38 Jana_in_Indiana: yes, I washed with a scrub brush & dish soap mixed with bleach, cut all my fingernails really short
05/18/05 20:17:38 jim: streptocarpus
05/18/05 20:17:58 Cheryl-TX: couldnt you wear gloves
05/18/05 20:18:05 Jana_in_Indiana: sure Jim, just get the protocol for the media for streps, it's not the same for all plants
05/18/05 20:18:28 Jana_in_Indiana: yes Cheryl, you don't HAVE to cut your nails, LOL
05/18/05 20:18:36 jim: i take it this doesnt cost an arm an a leg
05/18/05 20:18:43 adgjoan: how long ago did you do this?
05/18/05 20:18:55 Jana_in_Indiana: the basic kit now is around $80, I think
05/18/05 20:19:06 Jana_in_Indiana: last fall & again since then
05/18/05 20:19:23 Jana_in_Indiana: I kept a spray bottle of 10% bleach solution OR 70% alcohol (I’ve tried both & both are effective) inside the clean box to spray & wipe down EVERYTHING that my daughter passed through to me.
05/18/05 20:19:33 adgjoan: is there more than 1 application in a kit?
05/18/05 20:19:36 Cheryl-TX: what is in the kit, agar and nutrients
05/18/05 20:19:53 Jana_in_Indiana: yes, enough for several & you also get 1 free refill
05/18/05 20:19:55 Carol_SK: after you use the kit, is there stuff left over to use again, or do you start all over again with a new kit?
05/18/05 20:20:31 adgjoan: how many plants did you wind up with?
05/18/05 20:20:32 Jana_in_Indiana: yes, plus the hormones & PPM, plant preservative mixture to prevent mold & fungus
05/18/05 20:20:53 Jana_in_Indiana: Yes, Carol, lots of stuff to use again
05/18/05 20:21:13 jim: hate to run
05/18/05 20:21:19 jim: but i work tomorrow
05/18/05 20:21:20 Jana_in_Indiana: I don't have a total, but I think Pat will have to add another plant stand!
05/18/05 20:21:25 Jana_in_Indiana: g'nite Jim!
05/18/05 20:21:31 jim: will this chat be
05/18/05 20:21:31 Tony_IL: see you later Jim
05/18/05 20:21:34 Jana_in_Indiana: Once my hands & arms went into the clean box, they were not removed until everything was finished.
05/18/05 20:21:34 jim: listed
05/18/05 20:21:35 Carol_SK: nite Jim
05/18/05 20:21:43 sherry__(Mo): Nite Jim
05/18/05 20:21:57 Jana_in_Indiana: My first culture attempt was 24 individual cultures.  I’m proud to say we didn’t have a single instance of contamination in any of the 24 jars!
05/18/05 20:22:13 jim: i d like to hear about this later
05/18/05 20:22:15 jim: night
05/18/05 20:22:20 Jana_in_Indiana: Sterile procedure is almost automatic for me after all those years working in ICN (now called NICU), even though they were long ago.
05/18/05 20:22:29 jim Logs Out
05/18/05 20:22:42 Jana_in_Indiana: Even after sub-culturing (re-culturing into a different media for root formation) there is no contamination (rot, old, fungus, etc.)  My cultures are now in the rooting stage.
05/18/05 20:23:13 Jana_in_Indiana: My plan is to sterilize some Gel2Root & try transferring some of the cultures to it as a ‘baby’ step towards getting these tiny AV’s into soil.
05/18/05 20:23:18 adgjoan: do you have pics?
05/18/05 20:23:30 Tony_IL: how many plants do you expect a culture to yeild?
05/18/05 20:23:48 Jana_in_Indiana: my soil has compost & poop in it & is FAR from sterile, so I'll also have to make up some 1:1:1 mix & sterilize it
05/18/05 20:24:08 Jana_in_Indiana: easily 100, maybe more, I honestly don't know Tony
05/18/05 20:24:52 Cheryl-TX: and you didn 24 jars, do you mean 2400 babies
05/18/05 20:24:53 Jana_in_Indiana: yes Joan, but I can't post photos here, they are in the files on VR, folder named Tissue Culture, last page
05/18/05 20:24:54 Carol_SK: sounds like an effective way to come up with LOTS of babies the same
05/18/05 20:25:03 adgjoan: so you could have 2400 plants?
05/18/05 20:25:48 Jana_in_Indiana: no, some of the cultures didn't grow at all, they didn't rot, didn't mold, but didn't form a callus & make babies, it happenss sometimes
05/18/05 20:26:50 Jana_in_Indiana: I meant a 100 or more total from that one day, probably 30 or so per jar, some have more , some less, I'll count later & let everyone know
05/18/05 20:27:17 Cheryl-TX: how long does it take
05/18/05 20:27:26 Jana_in_Indiana: the piece of leaf was only about 1/2 inch square!
05/18/05 20:27:45 Jana_in_Indiana: at least 6 weeks for the first time, depending on temps
05/18/05 20:28:02 Jana_in_Indiana: since these were buckeyes, I kept them cooler so they grew slower
05/18/05 20:28:24 Jana_in_Indiana: then you sub culture them into a new medium that allows rooting
05/18/05 20:28:49 Jana_in_Indiana: then once they are well rooted you transfer them to sterilized soil -- that's where I'm at now
05/18/05 20:29:53 Jana_in_Indiana: it went slower than I thought, these were supposed to be for Pat to use at convention on the banquet tables
05/18/05 20:30:06 Jana_in_Indiana: cooler temps caused it to be slower
05/18/05 20:30:15 adgjoan: what did you put them in to get well rooted?
05/18/05 20:30:45 Jana_in_Indiana: it's just another medium recipe, but different hormones to allow rooting
05/18/05 20:31:17 Jana_in_Indiana: the first medium doesn't allow rooting, it's for shoot formation, & the 2 different ones can't be mixed, it won't work
05/18/05 20:31:28 adgjoan: which Buckeyes did you use?
05/18/05 20:32:35 Jana_in_Indiana: Buckeye Evensong    Buckeye Melancholy Mood    Buckeye Ohio Friendship    Buckeye Ruby Tuesday
05/18/05 20:33:03 Vivian__tn: BED TIME GOOD NIGHT EVERYONE!!!
05/18/05 20:33:07 Jana_in_Indiana: Ohio Friendship I will have a HUGE bunch of!
05/18/05 20:33:12 Jana_in_Indiana: g'nite Vivian!
05/18/05 20:33:20 Vivian__tn Logs Out
05/18/05 20:33:24 Tony_IL: nite Vivian
05/18/05 20:34:04 Jana_in_Indiana: there's fertilizer in the 2nd medium, along with the hormones & agar
05/18/05 20:34:22 adgjoan: what are you going to do with all of them?
05/18/05 20:34:26 Clare_KS: Can you sterilize the jars and equipment in the dishwasher?
05/18/05 20:34:31 Charlene_in_OK: What is agar and where can u get it?
05/18/05 20:34:45 Jana_in_Indiana: Pat will get lots of them & I'll share bunches too
05/18/05 20:35:24 Jana_in_Indiana: no, to sterilize it takes a pressure cooker or microwave over, need boiling or steam with the lids on but not quite air tight
05/18/05 20:35:37 adgjoan: do you think this is better than setting leaves?
05/18/05 20:35:46 Jana_in_Indiana: agar is the gelling ingredient, can also use gelrite
05/18/05 20:35:55 Charlene_in_OK: Is the point of this TC to have a bunch of babies from one leaf?
05/18/05 20:36:02 Jana_in_Indiana: I don't sell anything Joan
05/18/05 20:36:06 heinrichthelma Logs in [68.226.107.114]
05/18/05 20:36:39 Clare_KS: Jana, does the kit have enough materials to do it over again?
05/18/05 20:36:51 Jana_in_Indiana: it's to get a bunch from a limited amount of plant tissue, like if you hybridize & only have 1 really beautiful AV, use TC & get bunches faster than rooting leaves
05/18/05 20:37:12 adgjoan: I want you to compare the TC method versis setting leaves in soil. not selling.
05/18/05 20:37:27 Jana_in_Indiana: Yes Clare, the kit has enough to repeat 1 or 2 more times & you get 1 free refill after that!
05/18/05 20:37:41 Jana_in_Indiana: it's all about nubmers Joan
05/18/05 20:37:49 Jana_in_Indiana: numbers*
05/18/05 20:37:55 Clare_KS: I'll have to have my daughter-in-law start saving baby food jars.
05/18/05 20:38:20 Jana_in_Indiana: right Clare, or offer to pay a day care center 5 cents a jar to save them
05/18/05 20:38:42 Cheryl-TX: it would be good to use the plants produced for projexct plants
05/18/05 20:38:51 adgjoan: great idea,jana.
05/18/05 20:39:00 Jana_in_Indiana: i leaf in soil  can give you maybe 5 or 10 babies, 1 leaf in TC can give you 50 to 100
05/18/05 20:39:07 Charlene_in_OK: Is the reason it has to be so sterile because you are using such a small piece of tissue?
05/18/05 20:39:11 Jana_in_Indiana: right cheryl!
05/18/05 20:39:47 Jana_in_Indiana: yes Charlene that & the fact that fungus & mold will THRIVE in the culture medium & kill the plants
05/18/05 20:40:07 Jana_in_Indiana: it's to keep the bad germs out!
05/18/05 20:40:15 Clare_KS: It sounds like fun. I love to experiment.
05/18/05 20:40:20 Jana_in_Indiana: me too!
05/18/05 20:40:34 Charlene_in_OK: Does the kit have all the information on how to do it too?
05/18/05 20:41:10 Jana_in_Indiana: yes, comes with a hand book & then you also get to use the members only site on Kitchen Culture Kits web site
05/18/05 20:41:38 Jana_in_Indiana: it's really simple, just go slow so you don't make mistakes, like getting the pH exactly right
05/18/05 20:41:53 Charlene_in_OK: How do you do that?
05/18/05 20:41:55 Jana_in_Indiana: & measuring exactly as it says
05/18/05 20:41:58 Cheryl-TX: how long is procedure from start to finish
05/18/05 20:42:31 Jana_in_Indiana: test papers come with the kit, then you adjust up or down with vinegar or baking soda -- all before you sterilize it
05/18/05 20:42:45 Jana_in_Indiana: for me it was about 3 hours, I'm slow!
05/18/05 20:43:21 Jana_in_Indiana: and the meduim remains a liquid till you cook it to sterilize it, then it's semisolid
05/18/05 20:43:39 sherry__(Mo): But you were succesful and that's what counts Jana!
05/18/05 20:43:51 Jana_in_Indiana: you cook it in the baby food jars with the lids on, but not tight - they;ll blow up if on tight!
05/18/05 20:44:12 Charlene_in_OK: So again, how many jars did you use each time, which took you three hours to do?
05/18/05 20:44:15 Jana_in_Indiana: thanks Sherry, I am proud of that!  And, I'll have load to share with everyone
05/18/05 20:44:27 Jana_in_Indiana: 24 each time
05/18/05 20:45:02 Jana_in_Indiana: so need at least 48, cause the original cultures are still in their jars when you make up the rooting medium you will place them into
05/18/05 20:45:39 Charlene_in_OK: Gotcha, so the second 12 are for the 2nd step?
05/18/05 20:45:50 Charlene_in_OK: Sorry I meant 24
05/18/05 20:45:50 Jana_in_Indiana: to be honest, I didn't even try to save ever last one when transfering to the rooting medium, there were so darned many!
05/18/05 20:46:08 adgjoan: what kind of jars did you use?
05/18/05 20:46:13 Jana_in_Indiana: 24 for the 1st step, 24 more for the second step
05/18/05 20:46:21 Jana_in_Indiana: 6 ounce baby food jars
05/18/05 20:46:31 Charlene_in_OK: At the 1st step what do they look like when you transfer to the new medium
05/18/05 20:46:38 Jana_in_Indiana: my next TC will be of bloom stalks from chimeras
05/18/05 20:47:05 Jana_in_Indiana: itsy bitsy sprouts, I had photos but lost them all in the system restore
05/18/05 20:47:17 Jana_in_Indiana: maybe 1/4 inch tall
05/18/05 20:47:42 Cheryl-TX: the photos you took were excellent
05/18/05 20:47:51 Charlene_in_OK: Are the sprouts out of a piece of the leaf or just out of the media
05/18/05 20:47:58 Jana_in_Indiana: they were easy to sererate, but my hands just got too tired to keep saving every one towards the end
05/18/05 20:48:03 adgjoan: that would be great if you could get a bloom stalk of the blue and green chimera from Libby ar bluebird greenhouse.
05/18/05 20:48:06 Jana_in_Indiana: thanks Charyl!
05/18/05 20:48:09 Jana_in_Indiana: Cheryl!
05/18/05 20:48:26 Charlene_in_OK: I mean are they coming out of a piece?
05/18/05 20:48:42 Jana_in_Indiana: thing is, you need to have the plant -- the cutting wether from a leaf or bloom stalk must be VERY fresh
05/18/05 20:48:59 Clare_KS: Jana, You said 5 or 10 from a leaf in soil...is it about the same in the Gel2Root?
05/18/05 20:49:11 Jana_in_Indiana: they were all attached to the piece of leaf square
05/18/05 20:49:37 Jana_in_Indiana: more like 10 most of the time with gel2root
05/18/05 20:50:32 Jana_in_Indiana: next time I use leaves, I'll use larger pieces & then only harvest the largest sprouts
05/18/05 20:51:00 Jana_in_Indiana: theoretically, you can get 1 sprout from each cell in the leaf cutting
05/18/05 20:51:31 Jana_in_Indiana: any more questions?
05/18/05 20:51:58 sherry__(Mo): Yep-when can you move next door to me Jana-LOL
05/18/05 20:52:09 Clare_KS: Yeah, move to KC
05/18/05 20:52:12 Jana_in_Indiana: http://www.kitchenculturekit.com/Catalog.htm
05/18/05 20:52:12 sherry__(Mo): We'd have so much fun!
05/18/05 20:52:13 Charlene_in_OK: No next to me
05/18/05 20:52:14 Tony_IL: you said you had 2 people. Could it be done by 1 person?
05/18/05 20:52:25 Jana_in_Indiana: that's the link to the basic kit
05/18/05 20:52:31 adgjoan: can you leave the leaf piece on the medium to produce more sprouts like we reuse a moma leaf?
05/18/05 20:52:53 Jana_in_Indiana: it's done by 1 person all the time Tony, just need to get really organized before you start
05/18/05 20:52:54 Clare_KS: Can you resterilize and reuse the medium?
05/18/05 20:53:26 Jana_in_Indiana: no Joan, you couldn't even recognize it as a piece of leaf after it's calloused & has sprouts
05/18/05 20:53:59 Sarah Logs Out
05/18/05 20:54:22 Charlene_in_OK: So these 1/ " sprouts you divide and move to the second jars, or you seperate after that step?
05/18/05 20:54:27 Jana_in_Indiana: I haven't & don't think I'd want to waste the time trying it & risk having everything die, or not grow -- the plants absorb the hormones so they wouldn't be at the same levels of concentration
05/18/05 20:54:57 paula0103: Thanks Jana - must go.
05/18/05 20:55:09 Jana_in_Indiana: yes, when subcultiring into the rooting medium, you seperate them as much as possible
05/18/05 20:55:12 paula0103 Logs Out
05/18/05 20:55:15 Jana_in_Indiana: g'nite Paula!
05/18/05 20:55:40 Charlene_in_OK: Thanks so much for your great info Jana
05/18/05 20:55:50 sherry__(Mo): I second that Jana
05/18/05 20:56:03 Charlene_in_OK: By the way how are your little gal Yorkies
05/18/05 20:56:10 Cheryl-TX: this was great, i can hardly wait till school is out and i can try it
05/18/05 20:56:12 Jana_in_Indiana: wonderful & spoiled!
05/18/05 20:56:19 Jana_in_Indiana: you're welcome!
05/18/05 20:56:21 adgjoan: thanks, Jana loads of great info
05/18/05 20:56:30 Jana_in_Indiana: you're welcome!
05/18/05 20:56:40 Charlene_in_OK: Miss Emma too, what joys though huh?
05/18/05 20:56:49 Jana_in_Indiana: is that it?  I'm done?
05/18/05 20:56:54 Jana_in_Indiana: right charlene!
05/18/05 20:57:08 adgjoan: nitie nite
05/18/05 20:57:12 Cheryl-TX: yes, and you did a great job, Thanks!
05/18/05 20:57:12 Jana_in_Indiana: wow, that was fast & fairly painless, LOL
05/18/05 20:57:13 Charlene_in_OK: Thanks all. nitey nite
05/18/05 20:57:21 adgjoan Logs Out
05/18/05 20:57:23 Jana_in_Indiana: g'nite Charlene
05/18/05 20:57:28 Jana_in_Indiana: G'nite Joan!
05/18/05 20:57:40 Charlene_in_OK Logs Out
05/18/05 20:57:53 Jana_in_Indiana: I'll take more photos & puut them on my web shots
05/18/05 20:57:55 Clare_KS: Thanks, Jana :)
05/18/05 20:58:00 Carol_SK Logs Out
05/18/05 20:58:11 Jana_in_Indiana: that's why I got it, so if the computer crashes again, they'll still be there!
05/18/05 20:58:17 Cheryl-TX: the photos were great
05/18/05 20:58:17 Jana_in_Indiana: g'nite Clare
05/18/05 20:58:38 Jana_in_Indiana: only the last 2 photos were of my TC cheryl
05/18/05 20:58:50 Clare_KS Logs Out
05/18/05 20:59:04 Jana_in_Indiana: but you look at them & YOU tell me how many are in there!
05/18/05 20:59:28 Jana_in_Indiana: could be a new contest!  LOL  No one could cheat on that one!
05/18/05 20:59:31 sherry__(Mo): We won't see you-you'll be too busy potting up babies!!
05/18/05 20:59:52 sherry__(Mo): And building a few more rooms on the house-LOL
05/18/05 20:59:54 Jana_in_Indiana: never to busy for my groups, well, almost never!
05/18/05 21:00:29 Jana_in_Indiana: I'll keep maybe 3 or 4 of each, share others with buddies & give the rest to pat
05/18/05 21:00:29 sherry__(Mo): We're lucky to hsve you Jana!!
05/18/05 21:00:37 Cheryl-TX: well the photos were great
05/18/05 21:00:46 Jana_in_Indiana: thanks Sherry, but you're making me blush!
05/18/05 21:00:58 Jana_in_Indiana: & exaggerating!
05/18/05 21:01:31 Cheryl-TX: well i am going to go, am trying to finish my inventory
05/18/05 21:01:40 Jana_in_Indiana: g'nite cheryl!
05/18/05 21:01:47 sherry__(Mo): Good luck Cheryl-that's a job!
05/18/05 21:01:49 Cheryl-TX: got to do it quick, before i get more violets  lol
05/18/05 21:01:54 Jana_in_Indiana: send me a copy please?
05/18/05 21:02:00 Tony_IL: nite Cheryl'
05/18/05 21:02:01 Jana_in_Indiana: LOL
05/18/05 21:02:14 sherry__(Mo): I should go too-thanks for the great chat!!
05/18/05 21:02:24 Cheryl-TX: as soon i finish
05/18/05 21:02:26 Jana_in_Indiana: g'nite sherry!  Thank you!
05/18/05 21:02:29 Jana_in_Indiana: ok
05/18/05 21:02:33 sherry__(Mo): Take care everyone!!
05/18/05 21:02:35 Tony_IL: nite Sherry
05/18/05 21:02:36 sherry__(Mo) Logs Out
05/18/05 21:02:53 Cheryl-TX: so far i have 139 types and lots of duplicates
05/18/05 21:03:02 Jana_in_Indiana: LOL, me too!
05/18/05 21:03:15 Jana_in_Indiana: bet you don't have 50 to 100 of one kind!
05/18/05 21:03:29 Cheryl-TX: ok got to go, the is a dirty job but some one has to do it
05/18/05 21:03:36 Jana_in_Indiana: bye
05/18/05 21:03:45 Cheryl-TX: that is right,
05/18/05 21:03:49 Cheryl-TX Logs Out
05/18/05 21:04:28 Jana_in_Indiana: I need to go, I have suckers sitting on damp paper towels waiting to be repotted!
05/18/05 21:04:46 Jana_in_Indiana: Hey Tony, pass the test?
05/18/05 21:05:00 Jana_in_Indiana: or at least survive it OK?
05/18/05 21:05:17 Tony_IL: survived it
05/18/05 21:05:30 Jana_in_Indiana: whe  will you get the results?
05/18/05 21:05:44 Jana_in_Indiana: when*
05/18/05 21:05:44 Tony_IL: probably all on Monday
05/18/05 21:05:49 Jana_in_Indiana: ok
05/18/05 21:05:56 Jana_in_Indiana: ttyl!
05/18/05 21:06:26 Jana_in_Indiana: g'nite Thelma, Rachel, Tony & Violetangel
05/18/05 21:06:47 Tony_IL: nite Jana
05/18/05 21:07:12 Jana_in_Indiana Logs Out
05/18/05 21:08:12 Tony_IL: T-storms starting so I'm leaving. Later.........
05/18/05 21:08:36 Tony_IL Logs Out
05/18/05 21:18:47 heinrichthelma Logs Out
05/18/05 21:25:15 violetangel Logs Out
05/18/05 21:27:52 Becca,IN Logs in [71.98.76.32]
05/18/05 21:28:06 Becca,IN Logs Out
05/18/05 21:29:35 Rachel Logs Out
05/18/05 22:05:44 Becca,IN Logs in [71.98.76.32]
05/18/05 22:05:47 Becca,IN Logs Out
05/18/05 23:37:24 heinrichthelma Logs in [68.226.107.114]
05/18/05 23:37:32 heinrichthelma Logs Out